Microbiological analysis of cadmium-contaminated sediments during biostabilization with indigenous sulfate-reducing bacteria

Purpose

Sulfate-reducing bacteria (SRB) have received particular attention in the bioremediation of sediments contaminated with heavy metals. In this study, indigenous SRB were used to stabilize Cd in sediments spiked with different Cd concentrations (≤ 600 mg kg^?1).

Materials and methods

The study investigated the Cd leaching efficiency from sediments after 166 days (d) of biotreatment and assessed the bacterial community and bacteria relationship in sediments during SRB biostabilization.

Results and discussion

The study found that the Cd leaching efficiency of sediments was reduced by 18.1–40.3% (29.4 ± 8.7%) after 166 days of biotreatment. During the biostabilization, the bacterial community in sediments significantly changed, particularly after 61 days of biotreatment. At the family level, the identified dominant bacteria (mean abundance > 3%) included Bacillaceae, norank Nitrospira, Anaerolineaceae, Nitrospinaceae, Streptococcaceae, and Hydrogenophilaceae. The study also speculated the complex relationships between these bacteria. The relative abundance of Desulfobacteraceae and Desulfobulbaceae in sediments was enhanced after biotreatment. Bacillaceae and Streptococcaceae may play a negative role in Cd biostabilization and inhibited SRB biological activity. However, Anaerolineaceae and Hydrogenophilaceae may have commensalism and mutualism relationships, respectively, with typical SRB. The presence of Nitrospinacea and norank Nitrospira may reduce the inhibitive effect of denitrifying bacteria on SRB, thereby exhibiting a positive effect on biologic sulfate reduction and Cd biostabilization.

Conclusions

Indigenous SRB treatment increased Cd stability in sediments and changed bacterial community. During SRB biostabilization, complex relationships between bacteria in sediments were speculated, including competition, syntrophism, and antagonism. These results provide insights for better regulating and controlling SRB biostabilization.

     

Effects of climate factors and soil properties on soil nutrients and elemental stoichiometry across the Huang–Huai–Hai River Basin,China

Purpose

Soil nutrients, elemental stoichiometry, and their associated environmental control play important roles in nutrient cycling. The objectives of this study were (1) to investigate soil nutrients and elemental stoichiometry, especially potassium and its associative elemental stoichiometry with other nutrients under different land uses in terrestrial ecosystems; (2) to discuss the impacts of climate factors, soil texture, and soil physicochemical properties; and (3) to identify the key factors on soil nutrient levels and elemental stoichiometry.

Materials and methods

Soil data, including pH, bulk density (BD), cation exchange capacity (CEC), volumetric water content (VMC), clay, silt and sand contents, total carbon (TC), nitrogen (TN), phosphorous (TP) and potassium (TK), available nitrogen (AN), phosphorus (AP), potassium (AK), and soil organic matter (SOM) under different land-use types, were collected, and their elemental stoichiometry ratios were calculated. Climate data including temperature, precipitation, relative humidity, wind speed, and evapotranspiration were collected. The least significant difference test and one-way analysis of variance were applied to investigate the variability of soil nutrients and elemental stoichiometry among land-use types; the ordinary least squares method and the general linear model were used to illustrate the correlations between soil nutrients, elemental stoichiometry, and soil properties or climate factors and to identify the key influencing factors.

Results and discussion

Woodlands had the highest SOM, TN, AN, and AK contents, followed by grasslands, croplands, and shrublands, while the TP and TK contents only varied slightly among land-use types. SOM, TN, AN, N/P, and N/K were strongly negatively correlated to soil pH (p <?0.05) and were strongly positively correlated to soil CEC (p <?0.05). For soil texture, only C/N was moderately negatively correlated to silt content but moderately positively correlated to sand content (p <?0.05). For climate factors, SOM, TN, AN, N/P, and N/K were significantly negatively correlated to evapotranspiration and temperature (p <?0.05), and the correlations were usually moderate. Soil pH explained most of the total variation in soil nutrients, and climate factors explained 5.64–28.16% of soil nutrients and elemental stoichiometry (except for AP (0.0%) and TK (68.35%)).

Conclusions

The results suggest that climate factors and soil properties both affect soil nutrients and elemental stoichiometry, and soil properties generally contribute more than climate factors to soil nutrient levels. The findings will help to improve our knowledge of nutrient flux responses to climate change while also assisting in developing management measures related to soil nutrients under conditions of climate change.

     

华南型黄瓜新品种‘燕青’

‘燕青’黄瓜是以雌性系86G为母本，自交系Z-1为父本配制而成的华南型新品种。植株长势较强，强雌型。瓜圆筒形，长20 cm，平均单瓜质量186 g，瓜色白绿色，脆嫩微甜。早熟性突出，耐阴，耐寒，丰产性强，平均总产量达70.74 t • hm-2。适宜重庆及相似生态区春保护地、露地栽培。     

瓠瓜(Lagenaria siceraria)白粉病抗性的全基因组关联分析

白粉病是瓜类作物重要病害之一。本研究采用苗期人工接种方法,研究了117份瓠瓜微核心种质对白粉病的抗性,并基于不同材料的病情指数和基因型数据,进行全基因组关联分析(genome-wide association analysis,GWAS)。利用2014年和2018年的抗病表型数据,分别鉴定到22个和13个与白粉病抗性相关的SNPs,其中2个SNPs位点在两年试验中同时被检测到。进一步研究发现不同瓠瓜材料的白粉病抗性与其携带有利等位变异数目成正相关。本研究为克隆瓠瓜抗白粉病相关基因、研究其抗病机制提供科学依据,促进了瓠瓜抗白粉病分子育种。     

软基水闸底板脱空动力学反演模型构建与试验验证

软基水闸极易因地基不均匀沉降、渗透变形等发生底板脱空现象，传统的无损检测方法难以在水下探测底板脱空，且不具实时性，其应用还存在一定局限性。该研究基于软基水闸室内物理模型，提出了一种软基水闸底板脱空动力学反演方法。首先，基于多参数变量的底板脱空范围数学模型，改进了反映软基水闸底板脱空参数与水闸动力学参数（模态参数）之间非线性映射关系的数学代理模型；其次，以水闸多测点多阶频率和振型变化率组合作为动力学敏感特征量，建立了软基水闸底板脱空参数反演的目标函数，并基于遗传算法对目标函数进行求解。最后，建立了软基水闸室内物理模型，并在模型中设置3种不同的底板脱空工况，采用软基水闸底板脱空动力学反演方法对软基水闸底板的脱空进行反演识别与模型验证。结果表明：基于动力学参数反演识别的水闸底板脱空区域趋势和脱空面积与模型实际脱空情况吻合较好，3种工况下水闸底板脱空面积反演结果与模型实际脱空面积的相对误差分别为7.47%、6.78%、6.90%，验证了该方法的可靠性，可望为软基水闸实际工程底板脱空隐患检测提供一种新的思路。     

内蒙古河套灌区农田土壤中微塑料的赋存特征

为阐明内蒙古河套灌区农田土壤中微塑料的赋存特征及其与覆膜年限、灌溉类型等的响应关系,该文采用田间取样与室内试验相结合的方法进行了系统研究,分析了研究区地膜覆膜现状,并考虑覆膜年限(覆膜5、10、20 a)及灌水方式(滴灌和畦灌)2个因子,探索了河套灌区农田土壤中微塑料的丰度、类型、颜色、粒径等赋存特征,并通过扫描电镜、能谱仪等对微塑料表面特征及表面附着物进行观察与分析。结果表明:内蒙古河套灌区不同覆膜年限下(5、10、20 a)土壤中微塑料平均丰度分别为2526.00、4352.80、6070.00个/kg,单层土的微塑料含量最大值(2133.50 ind/kg)出现在覆膜20 a的0~10 cm土层,最小值(678.00个/kg)出现在覆膜5 a的>20~30 cm土层;不同覆膜年限和灌溉类型影响土壤微塑料丰度,滴灌农田微塑料丰度值略大于畦灌农田,在不同土层深度上微塑料丰度值随土层深度增加逐渐减小;微塑料类型主要有纤维类(23.34%)、碎片类(26.31%)、薄膜类(38.57%)和颗粒类(11.78%)等4种,且薄膜类在不同土层占比都较高,微塑料颜色包括黑色、透明、绿色、红色、蓝色等,比例分别为24.56%、23.83%、19.34%、16.52%和15.75%,其中0~10 cm土层中微塑料以黑色为主,占比30.25%,在>10~20、>20~30 cm土层中微塑料以透明为主,占比30.15%和29.23%,粒径则随覆膜年限增加而呈逐渐减少趋势,粒径小于1 mm的微塑料居多,随着覆膜年限的增加,微塑料粒径在0~10、>10~20、>20~30 cm土层间的差异逐渐减小,且粒径大小与灌溉类型无显著关系(P>0.05);微塑料样品表面特征粗糙,呈现不规则孔隙,纤维类、薄膜类和碎片类微塑料均具有较多规则微小孔隙,而颗粒类微塑料表面孔隙则不规则且呈凹凸状,土壤中微塑料的多孔特性造成微塑料比表面积增大,进而增加对土壤中其他污染物和微生物的吸附;微塑料的表面孔隙附着有机体和污染物,表面存在稳定的铁氧化物、稀土元素等,并容易形成有机-无机复合污染效应。研究对于明晰微塑料在河套灌区土壤中的分布现状及危害具有重要意义。     

TRMM在海河流域南系的降水估算精度评价及其对SWAT模型的适用性

准确估算区域降水对水文过程评价和水资源管理意义重大。为评估TRMM 3B42V7降水产品在海河流域南系的估算精度及其在土壤和水评估模型(Soil and Water Assessment Tool,SWAT)中的适用性,利用28个气象站降水观测数据(2007-2016年)和101个雨量站观测数据(2010-2016年)开展研究。研究表明:站点尺度上,3B42V7降水产品对月降水估算的均方根误差小于15 mm,平均误差小于8.5 mm;在湿润季节的估算精度更好。流域尺度上,日降水估算精度较差,相关系数小于0.6。分区尺度上,3B42V7能够很好地捕捉到不同等级降水强度,但对微量降雨有所低估;山区和平原的年降水量均出现高估现象,平原区较为突出;此外,3B42V7能够较好地捕捉到研究区内极端降水的时间和空间分布。分2种情景进行水文模拟,利用月平均流量对模型进行校准和验证,在情景Ⅰ中,验证期模拟结果较好,决定系数在0.56~0.96之间,纳什效率系数在-11.09~0.94之间。TRMM 3B42V7可为海河流域及其类似区域的水资源管理提供参考。     

Two-year fluctuation of Cd bioavailability in a remediated rice field under different water managements

Purpose

Based on two consecutive years of field-scale trials, under different water managements, we illustrated the persistence of remediation effect of palygorskite on a Cd-polluted rice field.

Materials and methods

The Cd uptake by a plant, pH and Cd chemical extractability, available P/K, and extractable Zn/Cu in paddy soils were used to evaluate the influence of palygorskite on Cd immobilization and soil fertility index.

Results and discussion

In contrast to the 1st year, at 0–1.5% palygorskite applied dose in soils, 0.025 M HCl–extractable Cd in continuous flooding reduced by 12.1–19.0%, and that in wetting irrigation increased by 10.9–18.9% in the 2nd year (p?<?0.05). The toxicity characteristic leaching procedure Cd reduction of 3.0–11.4% and increase of 8.9–12.0% were obtained under above-mentioned water managements (p?<?0.05). Compared with the 1st year, at different clay additional concentrations, grain Cd in continuous flooding reduced by 7.0–11.3%, and that in wetting irrigation increased by 6.5–10.8% in the 2nd year (p?<?0.05). Although trace elements in clay treated soils declined, they had no influence on the grain yield due to a minimum value higher than the critical value of 1.5 mg kg^?1 for Zn and 2.0 mg kg^?1 for Cu. The available P in continuous flooding took on a maximum increase of 8.2% in the 2nd year (p?<?0.05).

Conclusions

Two consecutive years of field-scale in situ demonstration tests revealed that continuous flooding was a preferable water management regime for Cd immobilization using palygorskite in the rice field. There were no remarkable differences in extractable Zn/Cu between 2 years.

     

山西云顶山亚高山草甸优势种种间关系分析

为揭示山西云顶山亚高山草甸植物群落优势种的种间关系,本研究通过种间联结性和相关性测定,对其植物群落13个优势种78个种对的种间关系进行研究。结果表明:13个优势种间大部分种对的种间关联不显著,种间联结不紧密,说明山西云顶山亚高山草甸植物群落结构不很稳定,存在一定程度的生态退化风险;多数原生优势种在群落中占据主要位置,同时也出现少数非原生优势种优势明显,有逐渐取代典型原生优势种的逆行演替趋势,这是由于人类频繁的旅游扰动所致。因此,应当合理的控制旅游开发强度与游客数量,保持亚高山草甸生态系统的稳定与自我维持,以持续高效的维持云顶山亚高山草甸的经济及生态价值。     

Pharmacokinetics of xylazine after 2-, 4-, and 6-hr durations of continuous rate infusions in horses

Intravenous (i.v.) bolus administration of xylazine (XYL) (0.5 mg/kg) immediately followed by a continuous rate infusion (CRI) of 1 mg kg⁻¹ hr⁻¹ for 2, 4, and 6 hr produced immediate sedation, which lasted throughout the duration of the CRI. Heart rate decreased and blood pressure increased significantly (p > .05) in all horses during the first 15 min of infusion, both returned to and then remained at baseline during the duration of the infusion. Compartmental models were used to investigate the pharmacokinetics of XYL administration. Plasma concentration–time curves following bolus and CRI were best described by a one-compartment model. No differences were found between pharmacokinetic estimates of the CRIs for the fractional elimination rate constant (K_e), half-life (t_1/2e), volume of distribution (V_d), and clearance (Cl). Median and range were 0.42 (0.15–0.97)/hr, 1.68 (0.87–4.52) hr, 5.85 (2.10–19.34) L/kg, and 28.7 (19.6–39.5) ml min⁻¹ kg⁻¹, respectively. Significant differences were seen for area under the curve ( ) (p < .0002) and maximum concentration (C_max) (p < .04). This indicates that with increasing duration of infusion, XYL may not accumulate in a clinically relevant way and hence no adjustments are required in a longer XYL CRI to maintain a constant level of sedation and a rapid recovery.